LB-Agar - Powder according to Lennox

A7459

BC

Order No.	Quantity
A7459,1000KG	1	kg

additional product information (PDF)

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Specification

Specification				Download specification (PDF)
state of matter	Solid
usage	for Luria-Bertani Agar plates according to Lennox for cultivation of E. coli in molecular biology
HS-No.:	38210000
Storage:	RT
WGK:	1
Specification
Appearance after heating	clear, brown liquid
Gel strength	passes test
Composition:
Agar	15 g/L
Yeast extract	5 g/L
NaCl	5 g/L
Tryptone	10 g/L

Comment

Comment
The packaging sizes differ in terms of the amount: you can choose between packages in liter (L) or gram (g) and kilogram (kg), respectively. Liter packs dissolved in the corresponding volume will give the correct concentration. When using the gram and kilogram packs, aliquots of the powder have to be dissolved. To prepare one liter of LB-Agar according to Lennox, 35 g of powder are dissolved. Directions to prepare 1 liter medium/agar: Dissolve the powder in 900 ml of water; adjust pH to 7.0 with 5 N NaOH (approx. 0.2 ml), fill to 1000 ml and autoclave. Antibiotics and nutritional supplements should be added only after the solution has cooled to 50°C or below, sine many of them are heat-sensitive.

The packaging sizes differ in terms of the amount: you can choose between packages in liter (L) or gram (g) and kilogram (kg), respectively. Liter packs dissolved in the corresponding volume will give the correct concentration. When using the gram and kilogram packs, aliquots of the powder have to be dissolved. To prepare one liter of LB-Agar according to Lennox, 35 g of powder are dissolved.
Directions to prepare 1 liter medium/agar: Dissolve the powder in 900 ml of water; adjust pH to 7.0 with 5 N NaOH (approx. 0.2 ml), fill to 1000 ml and autoclave. Antibiotics and nutritional supplements should be added only after the solution has cooled to 50°C or below, sine many of them are heat-sensitive.