Order No. Quantity
A1666,0025 25 g
A1666,0100 100 g
A1666,1000 1 kg

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Specification

Specification Download specification (PDF)
state of matter Solid 
Melting point 91 - 94°C 
Formula C2H4INO 
M 184.96 g/mol 
CAS-No.: 144-48-9 
HS-No.: 29241900 
EC-No.: 205-630-1 
Storage: 2-8°C 
LGK: 10 - 13 
Disposal:
Hazard pictogram(s) Hazard pictogram(s) GHS07 
Hazard statement(s) H315-H319-H317-H335 
Precautionary statement(s) P280-P302+P352-P304+P340-P305+P351+P338 
Signal word Warning 
WGK:
May darken in storage! 
Specification  
Assay (HPLC) min. 99 % 
Water max. 6 % 

Literature

Literature
  (1)  Gurd, F.R. (1972) Methods Enzymol. 25, 424-438
Carboxymethylation.
  (2)  Lane, L.C. (1978) Anal. Biochem. 86, 655-664
A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis.
  (3)  Marrakchi, N. et al. (1995) Biochim. Biophys. Acta 1244, 147-156
Cerastocytin, a new thrombin-like platelet activator from the venom of the tunisian viper Cerastes cerastes.
  (4)  Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193
Preparation of extracts from plants.

Comment

Comment
Iodoacetamide and Iodoacetate are well suited for the investigation of the active center of enzymes with sulfhydryl groups in its active center. The reaction conditions for the carboxymethylation may vary with every protein, which shall be modified by the corresponding reagents. Especially the optimal pH value and the concentration of the reagent (iodoacetamide, iodoacetate, chloroacetamide, chloroacetic acid) may vary (see table II in ref. 1).
In SDS polyacrylamide gel electrophoresis, the oxidation of sulfhydryl-containing proteins during electrophoresis may become a problem. Oxidation may non-reproducibly change the mobility of these proteins. Relief may come through reduction with DTE and subsequent alkylation with iodoacetamide of the proteins before loading the gel (2).
Iodoacetamide is used as a protease inhibitor too. It is added to several protease inhibitor cocktails in a concentration of 1 - 5 mM. At a concentration of 4 mM, e. g. the activity of the serine protease Cerastocytin has been inhibitied by more than 95 % (3). Please note, that this inhibitor, like iodoacetate and N-Ethylmaleimide, will be inactivated by reducing agents such as DTT. Especially for the preparation of plant cell extracts, high concentrations of reducing agents have to be added to the homogenizing buffers (4).
Iodoacetamide may become yellowish upon longer storage!