Order No. Quantity
A1499,1000 1 kg
A1499,5000 5 kg
A1499,9025 25 kg

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Specification

Specification Download specification (PDF)
Synonym Aminoformamidine Chloride, Carbamidine Chloride, GuaHCl, Guanidinium Chloride 
state of matter Solid 
Solubility (20°C) 2000 g/L (H2O) 
Melting point 183 - 188°C 
Formula CH5N3 · HCl 
M 95.53 g/mol 
CAS-No.: 50-01-1 
HS-No.: 29252900 
EC-No.: 200-002-3 
Storage: RT 
LGK: 10 - 13 
Disposal:
Hazard pictogram(s) Hazard pictogram(s) GHS07 
Hazard statement(s) H302+H332-H315-H319 
Precautionary statement(s) P261-P280-P301+P312-P304+P340-P305+P351+P338-P501 
Signal word Warning 
WGK:
Specification  
Assay (titr.) min. 99 % 
pH (5 %; H2O; 25°C) 4.0 - 6.0 
Insoluble matter passes test 
Water max. 0.5 % 
Ammonium max. 0.05 % 
Fe max. 0.0005 % 
Pb max. 0.0005 % 
A (1 cm/50 % in water HPLC grade)  
260 nm max. 0.1 
280 nm max. 0.05 

Literature

Literature
  (1)  MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227
Isolation of RNA using guanidinium salts.
  (2)  Cockle, S.A. et al. (1978) J. Biol. Chem. 253, 8019-8026
Resistance of lipophilin, a hydrophobic myelin protein, to denaturation by urea and guanidinium salts.

Comment

Comment
Guanidine hydrochloride denatures proteins and inhibits nucleases. It is applied at high concentrations for the isolation of non-degraded RNA (e. g. stock solution 7.5 M; ref. 1). β-Mercaptoethanol or DTT increase the denaturing activity of guanidine hydrochloride, since they break intramolecular disulfide bonds. Guanidine thiocyanate acts even stronger than guanidine hydrochloride as a denaturant and is used for 'valuable' samples/assays. Strongly hydrophobic proteins, like the membrane protein lipophilin will not be denatured by guanidine hydrochloride (2).