Order No. Quantity
A2252,0100 100 g
A2252,9025 25 kg

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Specification

Specification Download specification (PDF)
state of matter Solid 
additional product description non-ionic, synthetic polymere of sucrose 
M approx. 400000 g/mol 
CAS-No.: 26873-85-8 
HS-No.: 39072020 
EC-No.: 200-334-9 
Storage: RT 
LGK: 10 - 13 
WGK:
® registered trademark Pharmacia Biotech 
Specification  
Solubility (10 %; H2O) clear, colorless 
Heavy metals (as Pb) max. 0.001 % 
Loss on drying max. 3 % 

Literature

Literature
  (1)  Denhardt, D.T. (1966) Biochem. Biophys. Res. Com. 23, 641-646
A membrane-filter technique for the detection of complementary DNA.
  (2)  Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; p. 9.47-9.55, 6.12. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Comment

Comment
Ficoll® 400 is a component of Denhardt's solution (see A2248 and ref. 1, 2 [Page 9.47-9.55]) and several gel loading buffers (2 [Page 6.12]). The addition of Ficoll® 400, sucrose or glycerol increase the density of the loading buffers and facilitate the loading of the sample onto the gel.
Composition of loading buffers: I. 0.25 % Bromophenol blue, 0.25 % xylene cyanol FF, 15 % Ficoll® 400 in water (store at room temperature)
(according to ref. 2) II. alkaline loading buffer 300 mM NaOH, 6 mM EDTA, 18 % Ficoll® 400, 0.15 % Bromocresol green, 0.25 % xylene cyanol FF in water (store at +4°C)
Have a look at our 9 different loading buffers.