EGTA for molecular biology

A0878

BC

Order No.	Quantity
A0878,0025	25	g
A0878,0100	100	g

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Specification

Specification				Download specification (PDF)
Synonym	Ethylene Glycol-bis-(β-Aminoethyl Ether)-N,N,N',N'-Tetraacetic Acid, Ethylenedioxy-bis-(Ethylenenitrilo)-Tetraacetic Acid
state of matter	Solid
Melting point	240°C (dec.)
Formula	C₁₄H₂₄N₂O₁₀
M	380.35 g/mol
CAS-No.:	67-42-5
HS-No.:	29225000
EC-No.:	200-651-2
Storage:	RT
LGK:	10 - 13
WGK:	1
Specification
DNases/RNases/Proteases	not detectable
Assay (titr.)	min. 99 %
Residue on ignition	max. 0.1 %
Heavy metals (as Pb)	max. 0.001 %
Water	max. 1.0 %
Fe	max. 0.001 %

Literature

Literature
(1) Berman, M.C. (1982) J. Biol. Chem. 257, 1953-1957 Stimulation of calcium transport of sarcoplasmic reticulum vesicles by the calcium complex of ethylene glycol bis-(β-aminoethyl ether)-N,N'-tetraacetic acid. (2) Fuller, R.S. et al. (1989) Proc. Natl. Acad. Sci USA 86, 1434-1438 Yeast prohormone processing enzyme (KEX2 gene product) is a Ca²⁺-dependent serine protease. (3) Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193 Preparation of extracts from plants. (4) Liu, D. & Wu, R. (1999) BioTechniques 26, 258-261 Inhibition of DNase activity in plant nuclei with L-lysine and EGTA.

  (1)  Berman, M.C. (1982) J. Biol. Chem. 257, 1953-1957
Stimulation of calcium transport of sarcoplasmic reticulum vesicles by the calcium complex of ethylene glycol bis-(β-aminoethyl ether)-N,N'-tetraacetic acid.
  (2)  Fuller, R.S. et al. (1989) Proc. Natl. Acad. Sci USA 86, 1434-1438
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca²⁺-dependent serine protease.
  (3)  Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193
Preparation of extracts from plants.
  (4)  Liu, D. & Wu, R. (1999) BioTechniques 26, 258-261
Inhibition of DNase activity in plant nuclei with L-lysine and EGTA.

Comment

Comment
EGTA is a calcium specific chelator and can be used in the presence of Mg²⁺, since its affinity is 1000 times lower for Mg²⁺. EGTA inhibits metallo proteases at a concentration of 1 to 10 mM and is part of many inhibitor cocktails (3). The DNase from plant nuclei is efficiently inhibited by a combination of 160 mM L-lysine and 4 mM EGTA, enabeling to isolate DNA fragments with sizes of mega bases (4) Solutions of EGTA are prepared with 1 g EGTA in 10 ml of 1 M NaOH and filled up to 100 with ddH₂O (corresponds to a 26,29 mM solution).

EGTA is a calcium specific chelator and can be used in the presence of Mg²⁺, since its affinity is 1000 times lower for Mg²⁺. EGTA inhibits metallo proteases at a concentration of 1 to 10 mM and is part of many inhibitor cocktails (3). The DNase from plant nuclei is efficiently inhibited by a combination of 160 mM L-lysine and 4 mM EGTA, enabeling to isolate DNA fragments with sizes of mega bases (4)
Solutions of EGTA are prepared with 1 g EGTA in 10 ml of 1 M NaOH and filled up to 100 with ddH₂O (corresponds to a 26,29 mM solution).