Bis-Tris for buffer solutions

A1025

BC

Order No.	Quantity
A1025,0250	250	g
A1025,0500	500	g
A1025,1000	1	kg
A1025,9025	25	kg

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Specification

Specification				Download specification (PDF)
Synonym	Bis-(2-Hydroxyethyl)-Imino-tris-(Hydroxy Methyl)-Methane
state of matter	Solid
useful pH range	pH 5.8 - 7.2
pK_a (20°C)	6.4
Melting point	102 - 105°C
Formula	C₈H₁₉NO₅
M	209.24 g/mol
CAS-No.:	6976-37-0
HS-No.:	29221900
EC-No.:	230-237-7
Storage:	RT
LGK:	10 - 13
WGK:	1
Specification
Assay (titr.)	min. 99 %
pH (1 %; H₂O)	8.8 - 9.6
Heavy metals (as Pb)	max. 0.0003 %
Loss on drying	max. 1 %
Fe	max. 0.0005 %
A (1 cm/0.1 M in H₂O)
280 nm	max. 0.04
340 nm	max. 0.02

Literature

Literature
(1) Massie, B. et al. (1995) Bio/Technology 13, 602-608 Improved vector for the production of HSV-ribonucleotide reductase R1 and R2. (2) Merabet, E. & Ackers, G.K. (1995) Biochemistry 34, 8554-8563 Calorimetric analysis of the lcI-repressor binding to the DNA operator site. (3) Petri, V. et al. (1995) Biochemistry 34, 9977-9984 Characterization of the binding of the TATA-binding factor to adenovirus E4 promoter. (4) Scopes, R.K. ed. (1994) Protein Purification (Principles and Practice) 3rd Edition, p. 352; Springer-Verlag.

  (1)  Massie, B. et al. (1995) Bio/Technology 13, 602-608
Improved vector for the production of HSV-ribonucleotide reductase R1 and R2.
  (2)  Merabet, E. & Ackers, G.K. (1995) Biochemistry 34, 8554-8563
Calorimetric analysis of the lcI-repressor binding to the DNA operator site.
  (3)  Petri, V. et al. (1995) Biochemistry 34, 9977-9984
Characterization of the binding of the TATA-binding factor to adenovirus E4 promoter.
  (4)  Scopes, R.K. ed. (1994) Protein Purification (Principles and Practice) 3rd Edition, p. 352; Springer-Verlag.

Comment

Comment
Bis-Tris is an important buffer for protein and nucleic acid systems. Additionally it is used as a substitute for cacodylic acid buffer systems (4). The working concentration, mentioned in the literature (ref. 1-3), is 10 - 25 mM.