Order No. Quantity
A1442,0025 25 g
A1442,0050 50 g

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Specification

Specification Download specification (PDF)
Synonym para-Nitrophenyl Phosphate Disodium Salt 6-hydrate 
state of matter Solid 
Melting point 195°C 
Formula C6H4NNa2O6P · 6H2
M 371.15 g/mol 
CAS-No.: 4264-83-9 
HS-No.: 29199000 
EC-No.: 224-246-5 
Storage: -20°C 
protected from light 
Shipment: wet ice 
LGK: 10 - 13 
Disposal:
WGK:
Specification  
Assay (HPLC) min. 98 % 
4-Nitrophenol max. 0.01 % 

Literature

Literature
  (1)  Lowry, O.H. (1951) Methods Enzymol. 4, 366-381
Micromethods for the assay of enzymes.
  (2)  Bretaudiere, J. & Spillman, T. (1984) Methods Enzym. Anal. 4, 75-82
Alkaline phosphatase, IFCC Reference method.

Comment

Comment
In both references the substrate solution is 16 mM 4-nitrophenyl phosphate (in 2-amino-2-methyl-1-propanol ref. 2).