AppliCations No. 4 Improving Quality of ELISA

Using ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA is required because there is no kit available with the right antibodies or the characteristics of the available kits such as their limits of detection are not appropriate. Ready-to-use ELISA kits from good suppliers may be stored for two years at 4°C without any problem. With home-made ELISA it is a completely different story. For any new measurement one has to coat a new plate, because after storage of some days the plates don’t perform as well as before.

Why is there such a great difference in storage between home-made ELISA and ELISA kits?
The reason is that in professional ELISA kit production the plates are not only blocked after coating, but also stabilised. This easy to perform process has been an industry standard for thirty years. For stabilisation of a plate one has to incubate with a coating stabiliser solution. It is just as simple as a “second blocking step”. But there were no such high quality stabiliser solutions freely available in low volumes for use in research lab until now. AppliChem now offers a product for use in every research lab in volumes starting as small as 50 ml, which is called the AppliCoat Plate Stabiliser. This stabiliser solution is easy-to-use and has a great advantage compared to almost any stabiliser used in industry. It gives better storage stability for coated antibodies and antigens than most other products do.

Recognise and avoid interference in Immunoassays
Antibodies are useful tools to specifically detect many substances. Methods like Enzyme-linked immunosorbent assays (ELISA), Enzyme immunoassays (EIA), Western blotting, Radio immunoassays (RIA), Protein-Arrays, Immunohistochemistry, and the immuno-polymerase chain reaction (Immuno-PCR) belong to this category of immuno detection technology. Even if they are highly specific, one feature they share is they need to be disturbed by unwanted cross-reactivities. By applying a new buffer: AppliChem's CrossDown™ Buffer, instead of the usually used buffers, most interfering effects can be avoided.

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