New solutions allow multiple reuse of valuable material. Silica matrices are a key technology for the purification of DNA. Today the rapid isolation of pure DNA samples is essential for a variety of molecular biology protocols in research and commercial applications. Products with silica matrices are of high quality and high value. Their major disadvantage is that they can only be used once because after elution substantial amounts of DNA remain attached to the silica matrix and the binding capacity is reduced. To solve this problem AppliChem GmbH, Darmstadt in cooperation with multiBIND GmbH, Cologne developed the first regeneration system for silica matrices. Two innovative solutions remove all nucleic acids and extraneous material from silica matrices and restore the original binding capacity. The system is commercially available under the name maxXbond offering cost savings of approx. 70% with this unique regeneration technology.
This is the very first publication on the complete, DNA-free (contamination-free) regeneration of Silica matrices for the isolation of nucleic acids with maxXbond™! This is unique! Since the Silica matrices do not vary from supplier to supplier, regeneration of columns is manufacturer-independent.
The second publication on the complete, DNA-free (contamination-free) regeneration of Silica matrices for the isolation of nucleic acids with maxXbond™ was published in Nature methods! Since the Silica matrices do not vary from supplier to supplier, regeneration of columns is manufacturer-independent.
The complete information in terms of quality testing and function is available: Please view the separate maxXbond™ product information. This is the complete collection of data demonstrating the functionality and the performance of the maxXbond™ regeneration kit for pure silica matrices for the purification of nucleic acids. All methods employed, including gel electrophoresis, PCR and sequencing, could not detect any contamination after regeneration.
Most Midi prep DNA isolation kits and larger sized kits such as Maxi, Mega and Giga kits, employ another type of matrix. The silica-based anion exchange matrices (AX) are more complex compared to the pure silica matrix. Therefore, the recipes of the regeneration solutions and the protocol were adapted. The regeneration kit maxXbond™ AX is optimized for decontamination of silica-based anion exchange matrices (AX) and was developed in cooperation with multiBIND Biotech GmbH, Cologne, Germany. The publication is available here.