The contamination of cells with mycoplasma is a very common problem, even though it often goes unnoticed since no cloudiness appears in the cell culture. Nevertheless the contamination often causes biochemical changes as well as changes in the immunological properties of the cells. Since mycoplasma infected cells cannot always be discarded, many complicated methods have been suggested for the elimination of the mycoplasma. AppliChem is now offering a combination of antibiotics, which have been shown o be effective in the elimination of mycoplasma species that account for 90 % of the contamination found in cell culture. When used according to the following instructions, no cytotoxic effects will occur.
The problem of contamination in water baths, incubators and/or sterile work-benches is often a serious one, leading to extensive damage. To avoid contaminations regarding these delicate working aereas, AppliChem offers different product that effectively stop growing of fungi, mycoplasma and other bacteria:
Incubator-Clean solution prevents contamination with and growth of fungi (and spores), bacteria (and spores), mycoplasma and viruses (including HIV and Hepatitis B). The active ingredients are quarternary benzylammonium compounds, and the solution does not contain mercury, formaldehyde, phenol or alcohol. Furthermore, Incubator-Clean is non-toxic and biodegradable. It was also found it to be fully compatible with common work surfaces. Incubator-Clean is supplied in a one liter spray bottle. The water required to create the humidity is a source of contamination which disperses in the incubator. In order to disinfect the water we recommend Incuwater-Clean, which contains a disinfectant that does not cause damage to the stainless steel tray, is non-toxic, non-volatile, and extremely effective.
For disinfecting various kinds of water baths from bacteria and fungi Aquabator-Clean is intended. The active ingredient in Aquabator-Clean is safe to humans and does not cause any irritating effects to the skin when used in the recommended concentration. It is biodegradable.
This bochure (or flyer) informs about mycoplasma detection, treatment and prevention.
More current information about "Mycoplasma in Cell Culture" is provided in our flyer support&more No.1, where new products for detection and treatment were introduced: AppliChem provides the tools for detection and treatment of Mycoplasma for every cell culture laboratory. For the detection by microscopy we are offering the proven fluorescent dye DAPI (4',6-Diamidino-2-phenylindole dihydrochloride). In recent years the sensitive polymerase chain reaction (PCR) became a standard method for the detection of Mycoplasma contamination in biological samples such as mammalian cell cultures. The PCR is established in almost all life science labs either as standard PCR or real time/quantitative PCR. For your preferred setup, we offer three different kits to choose from: PCR Mycoplasma Test Kit, PCR Mycoplasma Test Kit II und qPCR Mycoplasma Test Kit.
Treatment of Mycoplasma Infections in Cell Cultures: AppliChem offers well-proven treatments to achieve reliable elimination of Mycoplasma infections from mammalian cell cultures, Myco-1&2 Set, Myco-3 and Myco-4. Myco-4 is a combination of a standard antibiotic and a biological reagent. In comparison to most bacteria, mycoplasma lack cell walls but are surrounded by a cytoplasmic membrane. This biological reagent integrates into the mycoplasma membrane and compromises its integrity. By the combination with a standard antibiotic, the effective dose of both, the reagent and the antibiotic, can be reduced to a minimum for lowest cytotoxicity, still causing a highly reliable and definite elimination of mycoplasma. These biophysical properties make the development of resistant strains very unlikely. Myco-4 leads to a permanent eradation of all mycoplasma-species. It is suitable for all permanent mammalian cell lines, also in combination with antibiotics for selection.