|Synonym||Refill Buffer Set for DNA Isolation Spin Kits|
|• Universal set of buffers for silica-based DNA binding columns|
|• all buffers can be stored at ambient temperature|
|• New formulation: Does not require addition of ethanol to wash buffer S5!|
|♦ use in combination with maxXbond™|
|♦ sufficient for at least 100, 250 and 1250 isolations, respectively|
|* maxXmore™ is a registered trademark of AppliChem GmbH.|
This is AppliChem's 5* Star buffer set (S1 - S5): An universal set of buffers for all DNA binding columns based on silica matrices for the recovery of DNA. All buffers are ready-to-use and can be stored at room temperature. The new formulation requires no addition of ethanol to wash buffer S5. Use of this buffer set is highly recommended, when columns have been regenerated with maxXbond™ (MB007) for multiple reuse.
Principle of the DNA isolation with silica matrices: DNA adsorbs to silica surfaces in the presence of high concentrations of salts. After modified alkaline lysis, the supernatant containing the plasmid DNA is loaded onto a spin colum. Plasmid DNA is adsorbed on the surface of the silica filter, while contaminants pass through without being adsorbed. After washing, pure DNA is eIuted in a low salt buffer (TE, Tris buffer) or water.