| Order No. | Quantity | |
|---|---|---|
A1008,0005
|
5 | g |
|
A1008,0025
|
25 | g |
A1008,0050
|
50 | g |
A1008,0100
|
100 | g |
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Specification
| Specification | ||||
|---|---|---|---|---|
| Synonym | Isopropyl β-D-Thiogalactopyranoside | |||
| state of matter | Solid | |||
| Melting point | 110 - 112°C | |||
| Formula | C9H18O5S | |||
| M | 238.30 g/mol | |||
| CAS-No.: | 367-93-1 | |||
| HS-No.: | 29329900 | |||
| EC-No.: | 206-703-0 | |||
| Storage: | 2 - 8°C | |||
| protected from light | ||||
| Shipment: | RT | |||
| LGK: | 10 - 13 | |||
| WGK: | 1 | |||
| Specification | ||||
| Assay (HPLC) | min. 99 % | |||
| α20°C/D; 1 %, H2O | -28.5° - -34.5° | |||
| pH (5 %; H2O) | 5.0 - 7.0 | |||
| Dioxane | max. 0.0001 % | |||
| Water (K.F.) | max. 1.0 % | |||
Literature
| Literature |
|---|
|
(1) Yansura, D.G. & Henner, D.J. (1984) Proc. Natl. Acad. Sci. USA 81, 439-443 Use of the E.coli lac repressor and operator to control gene expression in B. subtilis. (2) Cho, S. et al. (1985) Biochem. Biophys. Res. Com. 128, 1268-1273 Effect of IPTG concentration on the level of Lac-Operon induction in steady state E. coli. |
Comment
| Comment |
|---|
| The lactose-operon from E. coli is under negative control of the Lac-repressor protein, which specifically binds to the operator of the E. coli-genom. The most commonly used synthetic inductor of the Lac-operon is IPTG since it is both active at very low concentrations and not subject to enzymatic degradation. The final concentration differs from author to author (0.1 mM (2) - 1 mM (1) IPTG in LB-medium). For preparing a stock solution dissolve 100 mM or 500 mM IPTG in water or buffer, sterilize by filtration through a 0.22 μm filter and store at -20°C. |