Order No. Quantity
A0878,0005 5 g
A0878,0025 25 g
A0878,0100 100 g

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Specification

Specification Download specification (PDF)
Synonym Ethylene glycol-bis-(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid, Ethylenedioxy-bis-(ethylenenitrilo)-tetraacetic acid 
Melting point 240°C (dec.) 
Formula C14H24N2O10 
M 380.35 g/mol 
CAS-No.: 67-42-5 
HS-No.: 29225000 
EC-No.: 200-651-2 
Storage: RT 
LGK: 10 - 13 
Specification  
DNases/RNases/Proteases not detectable 
Assay (titr.) min. 99 % 
Residue on ignition max. 0.1 % 
Heavy metals (as Pb) max. 0.001 % 
Water max. 1.0 % 
Fe max. 0.001 % 

Literature

Literature
  (1)  Berman, M.C. (1982) J. Biol. Chem. 257, 1953-1957
Stimulation of calcium transport of sarcoplasmic reticulum vesicles by the calcium complex of ethylene glycol bis-(β-aminoethyl ether)-N,N'-tetraacetic acid.
  (2)  Fuller, R.S. et al. (1989) Proc. Natl. Acad. Sci USA 86, 1434-1438
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
  (3)  Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193
Preparation of extracts from plants.
  (4)  Liu, D. & Wu, R. (1999) BioTechniques 26, 258-261
Inhibition of DNase activity in plant nuclei with L-lysine and EGTA.

Comment

Comment
EGTA is a calcium specific chelator and can be used in the presence of Mg2+, since its affinity is 1000 times lower for Mg2+. EGTA inhibits metallo proteases at a concentration of 1 to 10 mM and is part of many inhibitor cocktails (3). The DNase from plant nuclei is efficiently inhibited by a combination of 160 mM L-lysine and 4 mM EGTA, enabeling to isolate DNA fragments with sizes of mega bases (4)
Solutions of EGTA are prepared with 1 g EGTA in 10 ml of 1 M NaOH and filled up to 100 with ddH2O (corresponds to a 26,29 mM solution).