|Aggregation no. (Na)||40|
|CMC (25°C)||9 x 10-5 mol/L|
|Classification: nonionic detergent|
|Melting range||40 - 42°C|
|LGK:||10 - 13|
|® registered trademark of Atlas Chemical Co.|
|Water||max. 4 %|
(1) Moore, S. & Stein, W.H. (1954) J. Biol. Chem. 211, 893-906
Procedure for the chromatographic determination of amino acids on 4 % cross-linked sulfonated polystyrene resins.
(2) Helenius, A. et al. (1979) Methods Enzymol. 56, 734-749
Review article: Properties of detergents.
Brij® 35 is a non-ionic detergent with a polyoxyethylene head group. As a rule, these detergents do not denature proteins, making them a useful tool in studying proteins in their native state. Proteins, that are composed of several subunits will not dissociate.
Preparation of a 0.5 % solution Brij® 35: Dissolve 0.5 g Brij® 35 in 100 ml hot water. If the detergent will not stay completely in solution at room temperature, enlarge the volume with water to 150 ml and use in the experiment 1.35X larger quantities (1).