Having information at your fingertips whenever you need it is one of the major foundations of productive work. Our heavyweight – the AppliChem General•Catalog 2011•2012 – claims nothing less than to simplify your lab work. Even though the digital age provides lots of new possibilities, we think that there is still no substitute for a printed work that provides valuable content. This enables you to always have product information ready at hand. Of course, you will learn all the news and trends from our website, a continually updated resource about our chemistry and capabilities.
A lot of things were accomplished in the last years. We set the course for the future – changes that will benefit you. We established a new product information management system and implemented the eCl@ss classification – currently our database holds 9000 different chemical substances. In addition, we planned new logistic facilities, further optimized manufacturing processes and implemented the GHS guidelines.
You can find all products arranged by product groups in our Product List by Product Groups.
Recombinant Proteins bioconfident grade.
What does that mean? Typically, growth factors and cytokines are produced in bacteria, yeast or other eukaryotic expression systems (e.g. insect cells, CHO cells). The organism selected for production, as well as the reagents for cultivation (components of media and additives) bear the risk of contamination by bacteria, viruses and TSE. Other components thereof, such as endogenously produced enzymes and other proteins, may contaminate the protein of interest. The endotoxin level of bacterially expressed growth factors is particularly difficult to minimize. bioconfident grade – reliable biologically safe: A newly developed expression system for recombinant proteins of animal origin uses the barley grain – endotoxinfree, antibiotic-free, serum-free, no endogenous growth factors, but natural protein folding and glycosylation.
Colorada – A New Generation of Fluorescent Labels.
The Colorada product line is a selection of the newest fluorescent labels available today. Dye structures were carefully chosen to provide top-notch brightness performances, improving bioanalytical assays to the highest level. Eight different basic structures with precise absorption and emission maxima were selected to cover the full working range of wavelengths, starting from 400 nm up to 850 nm. The Colorada structures are based on 4 different chemical classes: coumarin, dipyrromethene borondifluoride, acenaphthopyrrole and cyanine.
AppliCoat Plate Stabilizer – Improving Quality & Stability of ELISA.
Using ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA are required, perhaps because there is no kit available with the right antibodies or characteristics, such as the limits of detection are not appropriate. Ready-to-use ELISA kits from reputable suppliers may be stored for two years at 4°C without any problem. With home-made ELISA kits it is a completely different story. For any new measurement one has to coat a new plate, because after storage for some days the plates don’t perform as well as before. In commercial ELISA kit production, the plates are not only blocked after coating, but also stabilised. This easy-to-perform process has been an industry standard for thirty years. For stabilisation of a plate one has to incubate with a coating stabiliser solution. It is just as simple as a “second blocking step”. However there were no such high quality stabiliser solutions freely available in low volumes for use in the research lab until now. AppliChem now offers a product for use in every research lab in volumes starting as small as 50 ml, which is called the AppliCoat Plate Stabiliser (Cat. No. A7708).
Cell Proliferation Test Kit XTT.
Cell proliferation assays are widely used in cell biology for the study of growth factors, cytokines or media components. They are also applied in the screening of cytotoxic agents and lymphocyte activation. In order to determine the number of viable cells Cell Proliferation Kit XTT employs 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT). It is only in living cells that mitochondria are capable of reducing XTT to form an orange colored water soluble dye. Therefore, the concentration of the dye is proportional to the number of metabolically active cells. This test replaces the radioactive assays.
Size Exclusion Chromatography.
Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers. This system is also called gel filtration, typically with beads of dextran or agarose serving as gel matrix. Smaller molecules pass significantly slower through the column than larger molecules. Not to be confused with gel electrophoresis, there are big differences in terms of the separation principle. SEC does not require electric current and the sieving effect will not separate small molecules first. AppliChem‘s sizeexclusion chromatography columns are packed with AppliXchange-G25 M, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Typical applications are desalting, buffer exchange, purification of proteins and nucleic acids, and dye terminator removal.
Improving Separation During Electrophoresis: SeparateIT gels represent a novel gel matrix for DNA electrophoresis. Gel polymers are arranged in a conceptually different way, in accordance with a new theoretical model of gel electrophoresis. SeparateIT gels selectively retard the migration of large molecules, so that DNA bands remain sharp but are more spread out relative to each other. Thanks to this increased spacing, the resolving power of SeparateIT gels is at least twice that of any other gels, including polyacrylamide gels. Two DNA fragments in the 70-150 bp range that differ by 1 bp can be separated on SeparateIT gels that are 8 cm long.
now even more active – DNA decontamination as effective as bleach, but nonhazardous. We further developed the formulations of all liquid ExitusPlus™ reagents, resulting in a decontamination activity as high as bleach. The new reagents contain some alcohol (< 5 %) for improving the access of the active components to the surface, and a reduced concentration of color indicator.
We hope you enjoy browsing through the AppliChem General•Catalog 2011•2012.