Recombinant Proteins bioconfident grade: What does that mean?
Typically, growth factors and cytokines are produced in bacteria, yeast or other eukaryotic expression systems (e.g. insect cells, CHO cells). The organism selected for production, as well as the reagents for cultivation (components of media and additives) bear the risk of contamination by bacteria, viruses and TSE. Other components thereof, such as endogenously produced enzymes and other proteins, may contaminate the protein of interest. The endotoxin level of bacterially expressed growth factors is particularly difficult to minimize.

bioconfident grade - reliable biologically safe: A newly developed expression system for recombinant proteins of animal origin uses the barley grain. Barley endosperm storage cells, the nutritive tissue of the plant seeds, are particularly suitable for the production of recombinant proteins, since they are produced in a biochemically inert environment without endotoxins. The FDA classified barley as safe (G.R.A.S. Generally Recognised As Safe).

All bioconfident grade factors are carefully controlled to demonstrate and guarantee that plant-derived cytokines and growth factors show the same biological activity as commercially available proteins. The corresponding factor-specific bioactivity tests, such as cell proliferation assays, prove that all bioconfident grade factors are as active as factors produced in non-biologically-safe expression systems - frequently, results are even better! In addition, they are more compatible (e.g. cytotoxicity tests) than factors generated by other production techniques!

Theoretically, the following properties determine that the bioconfident grade growth factors and cytokines are ideal reagents for applications in cosmetics, pharma or the cultivation of stem cells.

• free from human or animal-infectious agents
• low pyrogenic and inflammatory activity
• free from other endogenous mammalian growth factors
• comparable in terms of eukaryotic glycosylation
• complete, natural protein folding, due to eukaryotic source
• free from animal products
• serum-free
• endotoxin-free
• no antibiotics
• low proteolytic activity

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